pcr buffer ii (Thermo Fisher)

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Thermo Fisher pcr buffer ii
Pcr Buffer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer+ii/TRIS-HCL/pm41781757-91-15-47
Average 99 stars, based on 1 article reviews

pcr buffer ii - by Bioz Stars, 2026-09

99/100 stars

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Polymerase Chain Reaction:

Article Title: Histological, ultrastructural, and single-cell profiling reveal immune-mediated remodeling in gallbladder inflammation.
Article Snippet: .. Each 25 μl reaction contained 0.3 μl of each primer, 2.5 μl of 10 × PCR buffer II (100 mM Tris–HCl, 500 mM KCl, pH 8.3), 2 μl of 15 mM MgCl2, 0.6 μl of 10 mM dNTP mix, 0.15 μl of AmpliTaq Gold polymerase (5 U/μl; Applied Biosystems), and nuclease-free water. .. Cycling conditions were as follows: 94 °C for 5 min, followed by 40 cycles of 94 °C for 20 s, 60 °C for 20 s, and 73 °C for 20 s, with a final extension at 72 °C for 7 min. PCR products were separated on 2% TAE agarose gels, visualized under UV illumination, and compared to a 100-bp DNA ladder (Invitrogen, 15,628,050).

Article Title: Methods of preparing nucleic acid libraries and compositions and kits for practicing the same
Article Snippet: .. Alternatively, cells can be heated to 65° C. for 10 minutes in water (Esumi et al., Neurosci Res 60(4):439-51 (2008)); or 70° C. for 90 seconds in PCR buffer II (Applied Biosystems) supplemented with 0.5% NP-40 (Kurimoto et al., Nucleic Acids Res 34(5):e42 (2006)); or lysis can be achieved with a protease such as Proteinase K or by the use of chaotropic salts such as guanidine isothiocyanate (U.S. ..

Article Title: Methylation detection and analysis of mammalian DNA
Article Snippet: .. Alternatively, cells can be heated to 65° C. for 10 minutes in water (Esumi et al., Neurosci Res 60(4): 439-51 (2008)); or 70° C. for 90 seconds in PCR buffer II (Applied Biosystems) supplemented with 0.5% NP-40 (Kurimoto et al., Nucleic Acids Res 34(5):e42 (2006)); or lysis can be achieved with a protease such as Proteinase K or by the use of chaotropic salts such as guanidine isothiocyanate (U.S. ..

Article Title: Single cell nucleic acid detection and analysis
Article Snippet: .. Alternatively, cells can be heated to 65° C. for 10 minutes in water (Esumi et al., Neurosci Res 60 (4):439-51 (2008)); or 70° C. for 90 seconds in PCR buffer II (Applied Biosystems) supplemented with 0.5% NP-40 (Kurimoto et al., Nucleic Acids Res 34 (5):e42 (2006)); or lysis can be achieved with a protease such as Proteinase K or by the use of chaotropic salts such as guanidine isothiocyanate (U.S. ..

Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors
Article Snippet: Genomic DNA contamination was eliminated using DNase-I (79254, Qiagen) and a reverse transcription was done for 50 min at 42 ◦ C using quantiscript reverse transcriptase (205313, Qiagen), with random hexamer primer. .. The subsequent PCR was performed by adding 1 μl cDNA, 0.5 μl of each primer (20 pM; MWG Biotech, Ebersberg, Germany) (Supplemental Table ), 2.5 μl 10× PCR buffer II (100 mM Tris–HCl, 500 mM KCl, pH 8.3), 2 μl MgCl 2 (15 mM), 0.5 μl dNTP (10 mM each), 0.1 μl AmpliTaq Gold polymerase (5 U/μl; all reagents from Applied Biosystems, Darmstadt, Germany) and 17.9 μl H 2 O. ..

Article Title: Single cell nucleic acid detection and analysis
Article Snippet: .. Alternatively, cells can be heated to 65° C. for 10 minutes in water (Esumi et al., Neurosci Res 60(4):439-51 (2008)); or 70° C. for 90 seconds in PCR buffer II (Applied Biosystems) supplemented with 0.5% NP-40 (Kurimoto et al., Nucleic Acids Res 34(5):e42 (2006)); or lysis can be achieved with a protease such as Proteinase K or by the use of chaotropic salts such as guanidine isothiocyanate (U.S. ..

Article Title: Methods of producing nucleic acids using oligonucleotides modified by a stimulus
Article Snippet: .. Alternatively, cells can be heated to 65° C. for 10 minutes in water (Esumi et al., Neurosci Res 60(4):439-51 (2008)); or 70° C. for 90 seconds in PCR buffer II (Applied Biosystems) supplemented with 0.5% NP-40 (Kurimoto et al., Nucleic Acids Res 34(5):e42 (2006)); or lysis can be achieved with a protease such as Proteinase K or by the use of chaotropic salts such as guanidine isothiocyanate (U.S. ..

Lysis:

Article Title: Single cell nucleic acid detection and analysis
Article Snippet: .. Alternatively, cells can be heated to 65° C. for 10 minutes in water (Esumi et al., Neurosci Res 60(4):439-51 (2008)); or 70° C. for 90 seconds in PCR buffer II (Applied Biosystems) supplemented with 0.5% NP-40 (Kurimoto et al., Nucleic Acids Res 34(5):e42 (2006)); or lysis can be achieved with a protease such as Proteinase K or by the use of chaotropic salts such as guanidine isothiocyanate (U.S. ..

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